Departs from the Moonee Ponds Junction bus terminal and runs via Mt. Alexander Road, Buckley Street, Russell Street to Essendon Railway Station. Then via Russell Street, Napier Street, Hayes Road, Carnarvon Road, York Street, Napier Street, Loch Crescent, Fenacre Street, Lebanon Street, Eldorado Street, Streldon Avenue, Woolart Street, Mascoma Street, Lockheed Street, Boeing Road, Mascoma Street, Matthews Avenue, Dromana Avenue to Airport West Shoppingtown. Then via Louis Street, Moore Road, Louis Street, Marshall Road, Sexton Street, Parer Road, Etzel Street, Clydesdale Road, South Road, McNamara Avenue, Roberts Road, El Reno Crescent, Grange Road, Fullarton Road, Matthews Avenue to the terminus at the corner of Matthews Avenue and Keilor Road.

Route 501 from Niddrie to Moonee Ponds

Departs from the terminus at the corner of Matthews Avenue and Keilor Road and runs via Keilor and Grange Roads, then via the reverse of the route described above to the Moonee Ponds Junction bus terminal.